Contrary to some opinion mainly due to the word "Squadron" being in the title of both films, this movie is not a sequel to the same studio's 633 Squadron.

Most of the aerial flying scenes were filmed at the airfield at Royal Bovington Royal Air Force (RAF) Station in Hertfordshire, UK.

The Bouncing Bomb used in the film was a real weapon. Known as Highball (a smaller version of the Dambusting Upkeep bomb), it was originally designed by Dr. Barnes Wallis to be utilized against battleships, such as the Tirpitz. The Highball bomb weighed 1,280lb (580kg), carried 600lb (272kg) of explosive, and was small enough to be carried in tandem by a Mosquito fighter-bomber. Prior to release the bomb was imparted with a backward spin of 700-900 revolutions per minute, and dropped at high speed (360 mph (580km/h)) and low altitude (60 ft (18.2m)).

The exquisite Chateau de Charlon seen in the movie was portrayed by Minley Manor, a Grade 2 listed country manor house, near Yateley and Farnborough in Hampshire, England. Over the past twenty years it has been actually the officers' mess (No 3 Royal School of Military Engineering) of Gibraltar Barracks.

The film utilizes an oft-used storyline of the war movie genre which has two soldiers in love with the same girl.

The Mosquito Squadron of this movie's title refers to a World War II squadron of Mosquito airplanes which are more specifically described as RAF (Royal Air Force) de Havilland Mosquito fighter-bomber aircraft.

The whole pre-credits sequence in its entirety was taken from the studio's previous picture, Operation Crossbow. This movie would also utilize footage from the studio's earlier 633 Squadron, most noticably the shot of Munroe rolling on the ground to extinguish the flames on the back of his flying jacket, after rerturning from the recon mission.

This was not the first movie to feature "bouncing bombs". They were seen in the earlier British war movie, The Dam Busters.